Directed molecular assembly into a biocompatible photosensitizing nanocomplex for locoregional photodynamic therapy

Title
Directed molecular assembly into a biocompatible photosensitizing nanocomplex for locoregional photodynamic therapy
Authors
이용덕조홍준최미화Park, HoyongBang, Joona이상엽권익찬김세훈
Keywords
Methylene blue; Polymer nanoparticles; Directed molecular assembly; Photodynamic therapy; MDA-MB-231 breast cancer cell
Issue Date
2015-07
Publisher
Journal of controlled release
Citation
VOL 209, 12-19
Abstract
Methylene blue (MB), a water-soluble cationic dye widely used in the clinic, is known to photosensitize the generation of cytotoxic singlet oxygen efficiently, and thus, has attracted interest as a potential drug for photodynamic therapy (PDT). However, its use for the in vivo PDT of cancer has been limited due to the inherently poor cell/ tissue accumulation and low biological stability in the free molecular form. Here, we report a simple and biocompatible nanocomplex formulation ofMB (NanoMB) that is useful for in vivo locoregional cancer treatment by PDT. NanoMB particles were constructed through the self-assembly of clinically usable molecules (MB, fatty acid and a clinically approved polymer surfactant) directed by the dual (electrostatic and hydrophobic) interactions between the ternary constituents. The nanocomplexed MB showed greatly enhanced cell internalization while keeping the photosensitization efficiency as high as free MB, leading to distinctive phototoxicity toward cancer cells. When administered to human breast cancer xenograft mice by peritumoral injection, NanoMB was capable of facile penetration into the tumor followed by cancer cell accumulation, as examined in vivo and histologically with the near-infrared fluorescence signal of MB. The quintuple PDT treatment by a combination of peritumorally injected NanoMB and selective laser irradiation suppressed the tumor volume efficaciously, demonstrating potential of NanoMB-based PDT as a biocompatible and safe method for adjuvant locoregional cancer treatment
URI
http://pubs.kist.re.kr/handle/201004/50574
ISSN
01683659
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KIST Publication > Article
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