Timescale analysis for estimating upper limit perfusion rate in a microfluidic perfusion cell culture platform

Title
Timescale analysis for estimating upper limit perfusion rate in a microfluidic perfusion cell culture platform
Authors
맹준호정효은신현준김세훈이종철이재영정석이상엽
Issue Date
2015-10
Publisher
Microfluidics and Nanofluidics
Citation
VOL 19, NO 4, 777-786
Abstract
Proliferation characteristics of CHO-K1 cells were investigated under a variety of medium perfusion rate conditions in a microfluidic perfusion cell culture platform. Five microcavities of were adopted in order to minimize or isolate the shear effects on cell surfaces. Microchannels of serially connecting these microcavities created flow contractions and expansions repeatedly, resulting in two different diffusion and convection timescales through the platform. Average shear stresses on the bottom of microcavity were both numerically and analytically estimated, and medium flow was operated at rates where shear stress is below 2 mPa. Proliferation rates of CHO-K1 cells were investigated based both on population groups derived from the number of initially seeded cells and on the microcavity locations. Population groups showed minimal influences on proliferation rates, while proliferation rates increased clearly with medium perfusion rates. Strong effects of microcavity locations were observed on proliferation at . Such effects were analyzed by investigating the relationships of reaction, diffusion, and convection timescales associated with perfusion conditions. The ratio of diffusion timescale and convection timescale was suggested as a guideline to estimate the upper limit of perfusion rate in microfluidic perfusion cell culture platform.
URI
http://pubs.kist.re.kr/handle/201004/50593
ISSN
16134982
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KIST Publication > Article
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