Cathepsin B Imaging to Predict Quality of Engineered Cartilage

Title
Cathepsin B Imaging to Predict Quality of Engineered Cartilage
Authors
Yhee, Ji YoungKim, Yong-Jin류주희윤홍열Chang, HyeyounPark, Jae HyungLee, HyukjinJang, Hyon-SeokJeong, Unyong김광명강선웅
Keywords
cartilage tissue engineering; cathepsin B; diagnosis; molecular imaging; transplantation
Issue Date
2015-09
Publisher
Macromolecular bioscience
Citation
VOL 15, NO 9, 1224-1232
Abstract
Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes.
URI
http://pubs.kist.re.kr/handle/201004/50597
ISSN
16165187
Appears in Collections:
KIST Publication > Article
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