Multifunctional nanoparticles for gene delivery and spinal cord injury

Title
Multifunctional nanoparticles for gene delivery and spinal cord injury
Authors
곽소정구희범윤여민이지영이혜영Yoon, Do Heum김광명하윤
Keywords
methylprednisolone; chitosan; spinal cord injury; gene delivery
Issue Date
2015-11
Publisher
Journal of biomedical materials research. Part A
Citation
VOL 103, NO 11, 3474-3482
Abstract
Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, p-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI.
URI
http://pubs.kist.re.kr/handle/201004/50599
ISSN
15493296
Appears in Collections:
KIST Publication > Article
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