Engineered Proteinticles for Targeted Delivery of siRNA to Cancer Cells

Title
Engineered Proteinticles for Targeted Delivery of siRNA to Cancer Cells
Authors
이은정이소진강윤식류주희권구철조은지이지영권익찬김광명이지원
Issue Date
2015-02
Publisher
Advanced functional materials
Citation
VOL 25, NO 8, 1279-1286
Abstract
Considering the problems of small interfering RNA (siRNA) delivery using traditional viral and nonviral vehicles, a new siRNA delivery system to enhance effi ciency and safety needs to be developed. Here human ferritin-based proteinticles are genetically engineered to simultaneously display various functional peptides on the surface of proteinticles: cationic peptide to capture siRNA, tumor cell targeting and penetrating peptides, and enzymatically cleaved peptide to release siRNA inside tumor cell. In the in vitro treatment of poly-siRNA-proteinticle complex, both of the tumor cell targeting and penetrating peptides are important for effi cient delivery of siRNA, and the red fl uorescent protein (RFP) expression in RFP-expressing tumor cells is notably suppressed by the delivered siRNA with the complementary sequence to RFP mRNA. It seems that the human ferritin-based proteinticle is an effi cient, stable, and safe tool for siRNA delivery, having a great potential for application to in vivo cancer treatment. The unique feature of proteinticles is that multiple and functional peptides can be simultaneously and evenly placed and also easily switched on the proteinticle surface through a simple genetic modifi cation, which is likely to make proteinticles appropriate for targeted delivery of siRNA to a wide range of cancer cells.
URI
http://pubs.kist.re.kr/handle/201004/50608
ISSN
1616301X
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KIST Publication > Article
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