Dependency of Experimental Autoimmune Encephalomyelitis Induction on MOG35?55 Properties Modulating Matrix Metalloproteinase-9 and Interleukin-6
- Dependency of Experimental Autoimmune Encephalomyelitis Induction on MOG35?55 Properties Modulating Matrix Metalloproteinase-9 and Interleukin-6
- 서지은; 마흐법하산; 한준승; 김낙균; 이지은; 이강미; 박주형; 김호준; 손정현; 이재익; 권오승
- MOG35-55; EAE; MMP-9; IL-6; Property; Induction
- Issue Date
- Neurochemical research
- Experimental autoimmune encephalomyelitis (EAE) is commonly induced with myelin oligodendrocyte
glycoprotein (MOG)35–55; occasionally, EAE is not well induced despite MOG35–55 immunization. To confirm that EAE induction varies with difference in MOG35–55 properties, we compared three MOG35–55 from different commercial sources, which are MOG-A, MOG-B, and MOG-C. The peptides induced EAE disease with 100, 40, and 20 % incidence, respectively. Compared with others, MOG-A
showed higher peptide purity (99.2 %) and content (92.2 %) and presented a sheet shape with additional sodium and chloride chemical elements. In MOG-A-treated group, MMP-9 activity and IL-6 levels were considerably higher than the other groups in CNS tissues, and significantly increased VCAM-1, IFN-c, and decreased IL-4 were also shown compared to MOG-B- and/or MOG-C-treated group. In conclusion, the immunological and toxicological changes by the difference in MOG35–55 properties modulate EAE induction, and MOG35–55 which affects MMP-9 activity and IL-6 levels may be the most effective EAEinducing antigen. This study can be potentially applied by researchers using MOG35-55 peptide and manufacturers for MOG35-55 synthesis.
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