Dependency of Experimental Autoimmune Encephalomyelitis Induction on MOG35?55 Properties Modulating Matrix Metalloproteinase-9 and Interleukin-6

Title
Dependency of Experimental Autoimmune Encephalomyelitis Induction on MOG35?55 Properties Modulating Matrix Metalloproteinase-9 and Interleukin-6
Authors
서지은마흐법하산한준승김낙균이지은이강미박주형김호준손정현이재익권오승
Keywords
MOG35-55; EAE; MMP-9; IL-6; Property; Induction
Issue Date
2015-10
Publisher
Neurochemical research
Abstract
Experimental autoimmune encephalomyelitis (EAE) is commonly induced with myelin oligodendrocyte glycoprotein (MOG)35–55; occasionally, EAE is not well induced despite MOG35–55 immunization. To confirm that EAE induction varies with difference in MOG35–55 properties, we compared three MOG35–55 from different commercial sources, which are MOG-A, MOG-B, and MOG-C. The peptides induced EAE disease with 100, 40, and 20 % incidence, respectively. Compared with others, MOG-A showed higher peptide purity (99.2 %) and content (92.2 %) and presented a sheet shape with additional sodium and chloride chemical elements. In MOG-A-treated group, MMP-9 activity and IL-6 levels were considerably higher than the other groups in CNS tissues, and significantly increased VCAM-1, IFN-c, and decreased IL-4 were also shown compared to MOG-B- and/or MOG-C-treated group. In conclusion, the immunological and toxicological changes by the difference in MOG35–55 properties modulate EAE induction, and MOG35–55 which affects MMP-9 activity and IL-6 levels may be the most effective EAEinducing antigen. This study can be potentially applied by researchers using MOG35-55 peptide and manufacturers for MOG35-55 synthesis.
URI
http://pubs.kist.re.kr/handle/201004/50640
ISSN
03643190
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KIST Publication > Article
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