Flow cytometric analysis of human lysozyme production in recombinant Saccharomyces cerevisiae
- Flow cytometric analysis of human lysozyme production in recombinant Saccharomyces cerevisiae
- Marvin S. Peterson; 김명동; 한기철; 김지현; 서진호
- flow cytometry; Saccharomyces cerevisiae; human lysozyme; cell cycle; secretion
- Issue Date
- Biotechnology and bioprocess engineering
- VOL 7, 52-55
- Flow cytometric techniques were used to investigate cell size, protein content and cell cycle behavior of recombinant Saccharomyces cerevisiae strains producing human lysozyme (HLZ).
Two different signal sequences, the native yeast MFα1 signal sequence and the rat α- amylase signal sequence, were used for secretion of HLZ. The strain containing the rat α-amylase signal sequence showed a higher level of internal lysozyme and lower specific growth rates. Flow cytometric analysis of the total protein content and cell size showed the strain harboring the native yeast signal sequence had a higher total protein content than the strain containing the rat α-amylase signal sequence. Cell cycle analysis indicated that the two lysozyme producing recombinant strains had an increased number of cells in the G2+M phase of the yeast cell cycle compared with the host strain SEY2102.
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