Optimization of fed-batch fermentation for xylitol production by Candida tropicalis

Title
Optimization of fed-batch fermentation for xylitol production by Candida tropicalis
Authors
김재한한기철고영호유연우서진호
Keywords
xylose; xylitol; Candida tropicalis; fed-batch fermentation
Issue Date
2002-06
Publisher
Journal of industrial microbiology & biotechnology
Citation
VOL 29, 16-19
Abstract
Xylitol, a functional sweetener, was produced from xylose by biological conversion using Candida tropicalis ATCC 13803. Based on a two-substrate fermentation using glucose for cell growth and xylose for xylitol production, fedbatch fermentations were undertaken to increase the final xylitol concentration. The effects of xylose and xylitol on xylitol production rate were studied to determine the optimum concentrations for fed-batch fermentation. Xylose concentration in the medium (100 g l –1 ) and less than 200 g l –1 total xylose plus xylitol concentration were determined as optimum for maximum xylitol production rate and xylitol yield. Increasing the concentrations of xylose and xylitol decreased the rate and yield of xylitol production and the specific cell growth rate, probably because of an increase in osmotic stress that would interfere with xylose transport, xylitol flux to secretion to cell metabolism. The feeding rate of xylose solution during the fed-batch mode of operation was determined by using the mass balance equations and kinetic parameters involved in the equations in order to increase final xylitol concentration without affecting xylitol and productivity. The optimized fed-batch fermentation resulted in 187 g l –1 xylitol concentration, 0.75 g xylitol g xylose– 1 xylitol yield and 3.9 g xylitol l –1 h–1 volumetric productivity.
URI
http://pubs.kist.re.kr/handle/201004/50648
ISSN
13675435
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KIST Publication > Article
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