Sensitivity of anabolic steroids based on GC-EI/MS, GC-EI/MS/MS, LC-ESI/MS/MS, LC-Ag+CIS/MS/MS and GC-ESI/MS/MS in doping control analysis

Title
Sensitivity of anabolic steroids based on GC-EI/MS, GC-EI/MS/MS, LC-ESI/MS/MS, LC-Ag+CIS/MS/MS and GC-ESI/MS/MS in doping control analysis
Authors
차은주김소희김희원김호준권오승이재익
Issue Date
2015-02
Publisher
33th Manfred Donike Workshop
Abstract
Anabolic steroids are important classes of doping agents and frequently used. Various researches were performed to improve the sensitivity and selectivity in steroids analysis using gas chromatography (GC) with electron impact ionization (EI) source or liquid chromatography (LC) with electrospray ionization (ESI) mass spectrometry (MS). In our previous reports (29-32th Manfred Donike Workshop, 2011-2014), we reported the new analytical techniques for simultaneous determination of steroids. In detail, steroids analysis using GC with soft ionization such as electrospray (ESI) and atmospheric-pressure chemical ionization (APCI) as novel ionization tool were investigated. Also silver ion coordination ionspray (Ag+CIS) method was developed as highly sensitive method for steroid analysis. In this study, we compared the sensitivity of the anabolic steroids using various instruments and methods (GC-EI/MS, GC-EI/MS/MS, LC-ESI/MS/MS, GC-ESI/MS/MS and LC-Ag+CIS/MS/MS). Sample preparation, chromatographic and MS parameters such as gradient condition, qualifier ion, SRM transition, collision energy and so on were optimized, respectively. The sensitivity of analytical method was evaluated by limit of detection (LOD) in standard spiked urine samples
URI
http://pubs.kist.re.kr/handle/201004/50665
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KIST Publication > Conference Paper
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