Integrated micro-optofluidic platform for real-time detection of airborne microorganisms

Title
Integrated micro-optofluidic platform for real-time detection of airborne microorganisms
Authors
최정안강미란정재희
Keywords
Bioaerosols; Microfluidics; Optofluidics; Real-time detection; Airborne microorganisms
Issue Date
2015-11
Publisher
Scientific Reports
Citation
VOL 5, NO 15983, 1-10
Abstract
We demonstrate an integrated micro-optofluidic platform for real-time, continuous detection and quantification of airborne microorganisms. Measurements of the fluorescence and light scattering from single particles in a microfluidic channel are used to determine the total particle number concentration and the microorganism number concentration in real-time. The system performance is examined by evaluating standard particle measurements with various sample flow rates and the ratios of fluorescent to non-fluorescent particles. To apply this method to real-time detection of airborne microorganisms, airborne Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis cells were introduced into the micro-optofluidic platform via bioaerosol generation, and a liquid-type particle collection setup was used. We demonstrate successful discrimination of SYTO82-dyed fluorescent bacterial cells from other residue particles in a continuous and real-time manner. In comparison with traditional microscopy cell counting and colony culture methods, this micro-optofluidic platform is not only more accurate in terms of the detection efficiency for airborne microorganisms but it also provides additional information on the total particle number concentration.
URI
http://pubs.kist.re.kr/handle/201004/50677
ISSN
20452322
Appears in Collections:
KIST Publication > Article
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