Rapid and sensative determination of HIV-1 virus based on surface enhanced raman spectroscopy

Title
Rapid and sensative determination of HIV-1 virus based on surface enhanced raman spectroscopy
Authors
이진호김병찬오병근최정우
Keywords
Au nanodot; raman scattering; surface-enhanced raman spectroscopy; virus
Issue Date
2015-12
Publisher
Journal of biomedical nanotechnology
Citation
VOL 11, NO 12, 2223-2230
Abstract
In this study, rapid and sensitive detection of human immunodeficiency virus (HIV)-1 was performed based on immunoreactions with an Au nanodot fabricated indium tin oxide (ITO) substrate using surface-enhanced Raman spectroscopy (SERS). Highly ordered Au nanodots (ca. 20 nm) were electrochemically fabricated over a large surface area (20 mm x 10 mm) of an ITO substrate using a simple deposition method with Triton X-100. On the Au nanodot surface, monoclonal antibody fragments against gp120 were selectively bound by gold-sulfur interactions. Various concentrations (35 fg/mL to 350 pg/mL) of HIV-1 virus-like particles (HIV-1 VLPs) were used for the measurements. The presence of HIV-1 VLPs was rapidly (within 5 s) and successfully determined by SERS due to specific immunoreactions on the Au nanodots without the use of labeling probes. The results showed the possibility of using SERS-related methods as a new immunoassay for the study of biomolecular interactions and detection of low viral loads. Moreover, based on its high sensitivity and chemical specificity, SERS could be used as a promising clinical tool for detecting infectious small biological components.
URI
http://pubs.kist.re.kr/handle/201004/50712
ISSN
15507033
Appears in Collections:
KIST Publication > Article
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