Gomisin J inhibits oleic acid-induced hepatic lipogenesis by activation of the AMPK dependent pathway and inhibition of the hepatokine fetuin-A in HepG2 cells

Title
Gomisin J inhibits oleic acid-induced hepatic lipogenesis by activation of the AMPK dependent pathway and inhibition of the hepatokine fetuin-A in HepG2 cells
Authors
김명석아흐마드 란디남의정노주원
Keywords
Gomisin J; AMPK; fetuin-A; HepG2; LKB1
Issue Date
2015-11
Publisher
The 6th International Conference on Food Factors
Abstract
The aim of our study is to investigate the molecular mechanism of gomisin J from Schisandra chinensis on the oleic acid (OA)-induced lipid accumulation in HepG2 cells. Triglyceride (TG) assays, and Oil red O staining were used to determine lipid accumulation in the HepG2 cells. Gomisin J attenuated lipid accumulation in OA-induced HepG2 cells. It also suppressed the expression of lipogenic enzymes and inflammatory mediators and increased the expression of lipolytic enzymes in OA-induced HepG2 cells. Furthermore, the use of specific inhibitors and fetuin-A-siRNA and liver kinase B1 (LKB1)-siRNA transfected cells demonstrated that gomisin J regulated lipogenesis and lipolysis via inhibition of fetuin-A and activation of an AMP-activated protein kinase (AMPK)-dependent pathway in HepG2 cells. Our results showed that gomisin J suppressed lipid accumulation by regulating the expression of lipogenic and lipolytic enzymes and inflammatory molecules through activation of AMPK, LKB1, and Ca2+/calmodulin-dependent protein kinase II and inhibition of fetuin-A in HepG2 cells. It suggested that gomisin J has potential benefits in preventing and treating nonalcoholic fatty liver disease.
URI
http://pubs.kist.re.kr/handle/201004/50772
Appears in Collections:
KIST Publication > Conference Paper
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE