In Vivo Voltage-Sensitive Dye Imaging of Mammalian Cortex Using "Blue" Dyes
- In Vivo Voltage-Sensitive Dye Imaging of Mammalian Cortex Using "Blue" Dyes
- 브래들리 베이커; Xin Gao; Brian S. Wolff; Lei Jin; 래리코헨; Chun X. Bleau; J.-Y. Wu
- Issue Date
- Cold Spring Harbor Protocols
- VOL 게재예정, 게재예
- Optical recording of membrane potential allows simultaneous measurements to be taken from
many different locations in the nervous system. This is important in studies of the nervous system in which simultaneous activity can occur at the regional, cellular, and subcellular levels. New “blue” dyes, developed by Amiram Grinvald’s group, are a great advance for in vivo voltage-sensitive dye imaging of mammalian cortex. The blue dyes are excited by red light (630 nm) that does not overlap with light absorption of hemoglobin (510–590 nm). This virtually eliminates the heart pulsation artifact.
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