Effect of 1-Deoxy-D-Xylulose 5-Phosphate Synthase (DXS) Gene Overexpression in Fucoxanthin Biosynthesis of Marine Diatom Phaeodactylum tricornutum

Title
Effect of 1-Deoxy-D-Xylulose 5-Phosphate Synthase (DXS) Gene Overexpression in Fucoxanthin Biosynthesis of Marine Diatom Phaeodactylum tricornutum
Authors
정유진신복규권벼리판철호김상민
Keywords
haeodactylum tricornutum; 1-deoxy-d-xylulose 5-phosphate synthase; transformants; fucoxanthin; diatom
Issue Date
2015-10
Publisher
2015년도 한국응용생명화학회 천연물분과 심포지엄
Abstract
Phaeodactylum tricornutum is a diatom which contains fucoxanthin as a main carotenoid pigment. In algae, fucoxanthin is synthesized via the methylerythritol phosphate (MEP) and carotenoid pathway. However, the mechanism and regulation of fucoxanthin biosynthesis has remained unclear until now. In this study, we developed nuclear transformants of P. tricornutum by metabolic engineering in order to increase fucoxanthin biosynthesis. We assumed that 1-deoxy-D-xylulose 5-phosphate synthase gene of P. tricornutum (PtDXS) is the best target for the increase of fucoxanthin biosynthesis because this step has been known as initial regulation point of the MEP and carotenoid pathway in plant. After introducing PtDXS gene into P. tricornutum cells by using the bombardment technique, we obtained two stable transformants. RT-PCR result showed that transcript level of PtDXS gene in one transformant was significantly increased compared to wild type. However, fucoxanthin content of that transformant was not increased up to the level of PtDXS transcript change, but showed slight increase. Further studies are needed to demonstrate the correlation between DXS gene expression and fucoxanthin biosynthesis.
URI
http://pubs.kist.re.kr/handle/201004/50892
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KIST Publication > Conference Paper
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