Hexanal-induced Changes in microRNA-mRNA Interactions in A549 Human Alveolar Epithelial Cells
- Hexanal-induced Changes in microRNA-mRNA Interactions in A549 Human Alveolar Epithelial Cells
- 조윤; 임정희; 정승찬; 송미경; 류재천
- Aldehydes; Hexanal; MicroRNA; Target mRNA; Microarray; Calcium signaling
- Issue Date
- Toxicology and environmental health sciences
- VOL 7, NO 2, 143-159
- Although hexanal is considered to be a major air pollutant the correlation between hexanal and health risk is largely unknown. Identifying aldehyde toxicity in eukaryotic cells will be useful for preventing and treating environmental diseases and disorders. However, it is important to note that previous microRNA (miRNA) studies of hexanal have not yet identified the functional relationship between miRNA and mRNA. The aim of this study is to investigate the modulation
of the integrated miRNA-mRNA relationship following exposure to hexanal. A549 human alveolar epithelial cells were treated to a 20% inhibitory concentration (IC20) of hexanal for 48 h. The microarray analysis results showed that 6 miRNA were altered in the hexanal-exposed A549 cells. Integrated analysis of miRNA and mRNA expression profiles identified 445 miRNA-mRNA correlations. KEGG analysis of 445 putative target genes of hexanal-induced miRNAs indicated that 8 genes (ATP2B3, ATP2B4, CACNA1G, EDNRA, GRM5, ITPR2, ITPKB, PTGER3) are involved in the calcium signaling pathway, which plays an important role in Alzheimer’s and several inherited immunodeficiency diseases. Therefore, this study suggests the value of miRNA-mRNA networks in order to identify molecular regulation in response to hexanal exposure in an in vitro model. Furthermore, this approach provides insight into the potential biological process of toxicity and a better understanding of the toxicological mechanism of toxicants.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.