Detecting Changes in Arthritic Fibroblast-Like Synoviocytes Using Atomi c Force Microscopy

Title
Detecting Changes in Arthritic Fibroblast-Like Synoviocytes Using Atomi c Force Microscopy
Authors
이지욱옥명렬이상목임진익
Keywords
Atomic force microscopy; fibroblast-like synoviocyte; roughness of cellular surface; morphology; rheumatoid arthritis
Issue Date
2015-11
Publisher
Microscopy research and technique
Citation
VOL 78, NO 11, 982-988
Abstract
The morphological and quantitative differences between arthritic fibroblast-like synoviocytes (FLS) and normal FLS were determined as an effective diagnostic tool for rheumatoid arthritis (RA), and confirmed using atomic force microscopy (AFM). Collagen-induced arthritic (CIA) mice and normal mice were prepared and FLS were isolated by enzymatic digestion from the synovial tissue of sacrificed mice at 5-week and 8-week pathogenesis periods. Analysis of cell morphology using AFM revealed that the surface roughness around the nucleus and around the branched cytoplasm was significantly higher in CIA FLS (P<0.05) than that in normal FLS. In addition, the roughness of two different sites on the arthritic FLS increased with an increase in the duration of pathogenesis. These results strongly suggest that AFM can be widely used as a diagnostic tool in cytopathology to detect the early signs of RA and various others diseases at the intercellular level. Microsc. Res. Tech. 78:982-988, 2015. (c) 2015 Wiley Periodicals, Inc.
URI
http://pubs.kist.re.kr/handle/201004/51171
ISSN
1059910X
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KIST Publication > Article
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