Investigation of the changes of biophysical/ mechanical characteristics of differentiating preosteoblasts in vitro

Title
Investigation of the changes of biophysical/ mechanical characteristics of differentiating preosteoblasts in vitro
Authors
라메쉬수하에리황민태김우준박귀덕
Keywords
Biophysical properties; Atomic force microscopy; Preosteoblasts; Osteogenesis; Focal adhesion; Young’s modulus
Issue Date
2015-12
Publisher
Biomaterials Research; 생체재료학회지
Citation
VOL 19, NO 24
Abstract
BACKGROUND: Topography, stiffness, and composition of biomaterials play a crucial role in cell behaviors. In this study, we have investigated biochemical (gene markers), biophysical (roughness), and biomechanical (stiffness) changes during the osteogenic differentiation of preosteoblasts on gelatin matrices. RESULTS: Our results demonstrate that gelatin matrices offer a favorable microenvironment for preosteoblasts as determined by focal adhesion and filopodia formation. The osteogenic differentiation potential of preosteoblasts on gelatin matrices is confirmed by qualitative (Alizarin red, von kossa staining, immunofluorescence, and gene expression) and quantitative analyses (alkaline phosphatase activity and calcium content). The biomechanical and biophysical properties of differentiating preosteoblasts are analyzed using atomic force microscopy (AFM) and micro indentation. The results show sequential and significant increases in preosteoblasts roughness and stiffness during osteogenic differentiation, both of which are directly proportional to the progress of osteogenesis. Cell proliferation, height, and spreading area seem to have no direct correlation with differentiation; however, they may be indirectly related to osteogenesis. CONCLUSIONS: The increased stiffness and roughness is attributed to the mineralized bone matrix and enhanced osteogenic extracellular matrix protein. This report indicates that biophysical and biomechanical aspects during in vitro cellular/extracellular changes can be used as biomarkers for the analysis of cell differentiation
URI
http://pubs.kist.re.kr/handle/201004/51208
ISSN
12264601
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KIST Publication > Article
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