Charge-selective membrane protein patterning with proteoliposomes

Title
Charge-selective membrane protein patterning with proteoliposomes
Authors
김희석이길용유수련정광환안태규이연희권오선박성진Kevin Kit Parker신관우
Issue Date
2015-02
Publisher
RSC advances
Citation
VOL 5, NO 7, 5183-5191
Abstract
A novel method to fabricate transmembrane protein (TP) embedded lipid bilayers using microcontact printing and applying proteoliposomes to different types of substrates, has been developed. The electrostatic interaction between the negatively charged proteoliposome and the substrate, which had been positively functionalized by microcontact printing, allowed the formation of TP-embedded, patterned lipid bilayers. The positively charged amino functional group on the substrate did effectively attract the negatively charged vesicles, inducing them to be adsorbed and subsequently ruptured to form a giant mosaic lipid bilayer, resulting in an immobilized TP-embedded lipid layer precisely on the targeted patterns, which were backfilled with a zwitterionic lipid bilayer. The rapid and highly selective recognition of the charged liposomes was visualized, and the biological functions of the TPs in the lipid matrix were also observed.
URI
http://pubs.kist.re.kr/handle/201004/51223
ISSN
20462069
Appears in Collections:
KIST Publication > Article
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