Insights into autophagosome maturation revealed by the structures of ATG5 with its interacting partners

Title
Insights into autophagosome maturation revealed by the structures of ATG5 with its interacting partners
Authors
Jun Hoe KimSeung Beom HongJae Keun LeeSisu HanKyung-Hye Roh이경은Yoon Ki KimEui-Ju ChoiHyun Kyu Song
Issue Date
2015-01
Publisher
Autophagy
Citation
VOL 11, NO 1, 75-87
Abstract
Autophagy is a bulky catabolic process that responds to nutrient homeostasis and extracellular stress signals and is a conserved mechanism in all eukaryotes. When autophagy is induced, cellular components are sequestered within an autophagosome and finally degraded by subsequent fusion with a lysosome. During this process, the ATG12-ATG5 conjugate requires 2 different binding partners, ATG16L1 for autophagosome elongation and TECPR1 for lysosomal fusion. In our current study, we describe the crystal structures of human ATG5 in complex with an N-terminal domain of ATG16L1 as well as an internal AIR domain of TECPR1. Both binding partners exhibit a similar a-helical structure containing a conserved binding motif termed AFIM. Furthermore, we characterize the critical role of the C-terminal unstructured region of the AIR domain of TECPR1. These findings are further confirmed by biochemical and cell biological analyses. These results provide new insights into the molecular details of the autophagosome maturation process, from its elongation to its fusion with a lysosome.
URI
http://pubs.kist.re.kr/handle/201004/51296
ISSN
15548627
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KIST Publication > Article
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