ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging

Title
ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging
Authors
Eunsoo LeeJungyoon ChoiYouhwa JoJoo Yeon KimYu Jin JangHye Myeong LeeSo Yeun KimHo-Jae LeeKeunchang ChoNeoncheol Jung허은미Sung Jin JeongCheil MoonYoungshik ChoeIm Joo RhyuHyun KimWoong Sun
Issue Date
2016-01
Publisher
Scientific Reports
Abstract
Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues, which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process times. Here, we present the rapid and highly reproducible ACTPRESTO (active clarity technique-pressure related efficient and stable transfer of macromolecules into organs) method that clears tissues or the whole body within 1 day while preserving tissue architecture and protein-based signals derived from endogenous fluorescent proteins. Moreover, ACT-PRESTO is compatible with conventional immunolabeling methods and expedites antibody penetration into thick specimens by applying pressure. The speed and consistency of this method will allow high-content mapping and analysis of normal and pathological features in intact organs and bodies.
URI
http://pubs.kist.re.kr/handle/201004/58416
ISSN
20452322
Appears in Collections:
KIST Publication > Article
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