Extensible Multiplex Real-time PCR of MicroRNA Using Microparticles

Title
Extensible Multiplex Real-time PCR of MicroRNA Using Microparticles
Authors
정승원김준선이동진오은해임화섭김광표최낙원김태송김상경
Keywords
분자진단; 다중마커 진단; 실시간 PCR; 입자기반 qPCR; miRNA; 패턴인식 입자식별
Issue Date
2016-03
Publisher
Scientific Reports
Citation
VOL 6, NO 22975
Abstract
Multiplex quantitative real-time PCR (qPCR), which measures multiple DNAs in a given sample, has received significant attention as a mean of verifying the rapidly increasing genetic targets of interest in single phenotype. Here we suggest a readily extensible qPCR for the expression analysis of multiple microRNA (miRNA) targets using microparticles of primer-immobilized networks as discrete reactors. Individual particles, 200~500 μm in diameter, are identified by two-dimensional codes engraved into the particles and the non-fluorescent encoding allows high-fidelity acquisition of signal in real-time PCR. During the course of PCR, the amplicons accumulate in the volume of the particles with high reliability and amplification efficiency over 95%. In a quick assay comprising of tens of particles holding different primers, each particle brings the independent real-time amplification curve representing the quantitative information of each target. Limited amount of sample was analyzed simultaneously in single chamber through this highly multiplexed qPCR; 10 kinds of miRNAs from purified extracellular vesicles (EVs).
URI
http://pubs.kist.re.kr/handle/201004/58770
ISSN
20452322
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE