Transdifferentiation of human endothelial progenitors into smooth muscle cells

Title
Transdifferentiation of human endothelial progenitors into smooth muscle cells
Authors
정영미HaYeun JiLeigh AtchisonZaozao Chen Zaozao ChenSyandan ChakrabortyGeorge A. TruskeyNicolas ChristoforouKam W. Leong
Keywords
Direct transdifferentiation; Direct reprogramming; Smooth muscle cell differentiation; Myocardin; Tissue-engineered blood vessel
Issue Date
2016-04
Publisher
Biomaterials
Citation
VOL 85, 180-194
Abstract
Access to smooth muscle cells (SMC) would create opportunities for tissue engineering, drug testing, and disease modeling. Herein we report the direct conversion of human endothelial progenitor cells (EPC) to induced smooth muscle cells (iSMC) by induced expression of MYOCD. The EPC undergo a cytoskeletal rearrangement resembling that of mesenchymal cells within 3 days post initiation of MYOCD expression. By day 7, the reprogrammed cells show upregulation of smooth muscle markers ACTA2, MYH11, and TAGLN by qRT-PCR and ACTA2 and MYH11 expression by immunofluorescence. By two weeks, they resemble umbilical artery SMC in microarray gene expression analysis. The iSMC, in contrast to EPC control, show calcium transients in response to phenylephrine stimulation and a contractility an order of magnitude higher than that of EPC as determined by traction force microscopy. Tissue-engineered blood vessels constructed using iSMC show functionality with respect to flow- and drug-mediated vasodilation and vasoconstriction.
URI
http://pubs.kist.re.kr/handle/201004/59084
ISSN
01429612
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KIST Publication > Article
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