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dc.contributor.author김수현-
dc.contributor.author정영미-
dc.contributor.author이보람-
dc.contributor.author무하매드 샤피크-
dc.contributor.author박종철-
dc.date.accessioned2016-05-24T08:25:15Z-
dc.date.available2016-05-24T08:25:15Z-
dc.date.issued2016-02-
dc.identifier.citationVOL 24, NO 2, 131-142-
dc.identifier.issn15985032-
dc.identifier.other46235-
dc.identifier.urihttp://pubs.kist.re.kr/handle/201004/59086-
dc.description.abstractTissue-engineered vascular scaffolds provide a promising solution for the replacement of diseased vascular structures. However, a major challenge lies in enhancing endothelialization, host cell ingrowth, and angiogenesis. In this study, we investigated the feasibility of developing a bio-tubular scaffold from human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVEC) co-cultured on electrospun poly(L-lactide-co-ε-caprolactone) membranes to address these issues. Confluent layers of HDFs stimulated the organization of HUVECs into capillary-like networks in an indirect contact (two-dimensional) co-culture on membranes. Bio-tubular scaffolds fabricated from co-cultured membranes were either grown statically in vitro or implanted subcutaneously in severe combined immunodeficient mice for up to 4 weeks for biocompatibility evaluation and functional performance. In vitro examination of co-cultures on scaffolds showed collagen remodeling and an improvement in biomechanical properties up to day 14. Morphological analysis of in vitro grown bio-tubular scaffolds revealed good attachment and growth of both cell types. After one month, co-cultured scaffolds in vivo showed higher infiltration of host cells and collagen remodeling as compared to the HDF-seeded grafts. After 4 weeks, thin continuous layers of endothelial cells and smooth muscle cells were formed as shown by staining with an antibody specific for CD31and α-actin (α-SMA). On the contrary, HDF-seeded scaffolds remained free of α-SMA-positive cells at all time points, whereas few CD31+ cells appeared after 4 weeks. Thus, co-cultured membranes provide a solution for enhancing endothelialization, tissue regeneration, and growth in bio-tubular scaffolds and may have broader applications in regenerative medicine.-
dc.publisherMacromolecular research-
dc.titleCharacterization and preparation of bio-tubular scaffolds for fabricating artificial vascular grafts by combining electrospinning and a co-culture system-
dc.typeArticle-
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