Isolation of extracellular vesicle from blood plasma using electrophoretic migration through porous membrane
- Isolation of extracellular vesicle from blood plasma using electrophoretic migration through porous membrane
- 강지윤; 곽노균; 허유희; 조시우; 조원주; 박재성
- Exosome; Purification; Separation; Nanoporous membrane; Body fluid
- Issue Date
- Sensors and actuators. B, Chemical
- VOL 233, 289-297
- We present a high-yield electro-migration method to isolate extracellular vesicles (EVs), which achieves ultracentrifugation (U/C)-level size exclusion from biological fluids (e.g., plasma). An electric field applied across a dialysis membrane with an appropriate pore size (30 nm), facilitates protein migration through the membrane, but captures EVs on the membrane surface. Compared to conventional procedures, this method shows up to 65% EV recovery estimated at the RNA level (∼7.9 times better than U/C) and up to 83.6% protein removal (residual protein amount is approximately half of the precipitate) in ∼30 min (∼9 times faster than U/C). With optimized working range of voltage and use of biochemically stable buffers, isolated EV are fully compatible with biological post-processes and assays.
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