Isolation of extracellular vesicle from blood plasma using electrophoretic migration through porous membrane

Title
Isolation of extracellular vesicle from blood plasma using electrophoretic migration through porous membrane
Authors
강지윤곽노균허유희조시우조원주박재성
Keywords
Exosome; Purification; Separation; Nanoporous membrane; Body fluid
Issue Date
2016-10
Publisher
Sensors and actuators. B, Chemical
Citation
VOL 233, 289-297
Abstract
We present a high-yield electro-migration method to isolate extracellular vesicles (EVs), which achieves ultracentrifugation (U/C)-level size exclusion from biological fluids (e.g., plasma). An electric field applied across a dialysis membrane with an appropriate pore size (30 nm), facilitates protein migration through the membrane, but captures EVs on the membrane surface. Compared to conventional procedures, this method shows up to 65% EV recovery estimated at the RNA level (∼7.9 times better than U/C) and up to 83.6% protein removal (residual protein amount is approximately half of the precipitate) in ∼30 min (∼9 times faster than U/C). With optimized working range of voltage and use of biochemically stable buffers, isolated EV are fully compatible with biological post-processes and assays.
URI
http://pubs.kist.re.kr/handle/201004/59222
ISSN
09254005
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KIST Publication > Article
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