Doxorubicin/heparin composite nanoparticles for caspase-activated prodrug chemotherapy

Doxorubicin/heparin composite nanoparticles for caspase-activated prodrug chemotherapy
권익찬김인산Nisar Ul KhaliqFebrina Carolina SandraDal Yong ParkJae Young LeeKeun Sang OhDongkyu KimYoungro ByunSang Yoon KimSoon Hong Yuk
Issue Date
VOL 101, 131-142
Caspase-activated prodrug chemotherapy is introduced and demonstrated using the composite nano particles (NPs), which deliver doxorubicin (DOX) and DEVD-S-DOX together to the tumor tissue. DEVD-S-DOX, DOX linked to a peptide moiety (DEVD), is a prodrug that is cleaved into free DOX by caspase-3 upon apoptosis. DEVD-S-DOX has no therapeutic efficacy, but it changes into free DOX with the expression of caspase-3. With the accumulation of the composite NPs in the tumor tissue by the enhanced permeation and retention (EPR) effect, a small exposure of DOX in the tumor. cells initiated apoptosis in a localized area of the tumor tissue, which induced caspase-3 activation. Cleavage of DEVD-S-DOX into free DOX by caspase-3 continued with repetitive activation of caspase-3 and cleavage of DEVD-S-DOX at the tumor site. The composite NPs were characterized with transmittance electron microscopy (TEM) and particle size analyzer. We then evaluated the nanoparticle drug release, therapeutic efficacy, and in vivo biodistribution for tumor targeting using a non-invasive live animal imaging technology and the quantification of DOX with high performance liquid chromatography. DOX-induced apoptosis-targeted chemotherapy (DIATC) was verified by in vitro/in vivo DEVD-S-DOX response to free DOX and cellular uptake behavior of the composite NPs with flow cytometry analysis. Significant antitumor efficacy with minimal cardiotoxicity was also observed, which supported DIATC for improved chemotherapy. (C) 2016 Elsevier Ltd. All rights reserved.
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