Genome analysis and influences of cofactors on syngas fermentation by C2~C6 acid/alcohol-producing Clostridium sp. JS66

Title
Genome analysis and influences of cofactors on syngas fermentation by C2~C6 acid/alcohol-producing Clostridium sp. JS66
Authors
이선미엄영순김경식최재표김경헌
Issue Date
2018-10
Publisher
2018 한국생물공학회 추계학술발표대회 및 국제심포지엄
Abstract
Syngas and steel waste gas including carbon monoxide, carbon dioxide, and hydrogen are widely studied to use in biological production of acid and alcohol using acetogens. Among them, a newly isolated acetogen Clostridium sp. JS66 identified as a noble species with 33 % of DNA-DNA hybridization value with Clostridium carboxidivorans P7 is able to convert syngas into nC2~nC6 acid/alcohol metabolites through the Wood-Ljungdahl pathway and chain elongation pathways. Essential genes encoding formate dehydrogenase (Fdh) converting CO2 into formate, hydrogenase forming a tight complex with formate hydrogenase (Hyt), and aldehyde oxidoreductase (AOR) synthesizing aldehyde from acetate were identified through the genome analysis and were compared with the genes in Acetobacterium woodii and C. autoethanogenum. As those enzymes are known to need a specific metal ion such as tungsten (W), molybdenum (Mo) and selenium (Se) as a cofactor, the effects of Mo, W, and Se on syngas fermentation were investigated and elucidated with the genomic analysis. Further Omics study would provide more information on metabolic pathways and energetic conservation mechanism of Clostridium sp. JS66.
URI
http://pubs.kist.re.kr/handle/201004/61825
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KIST Publication > Conference Paper
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