Genome analysis and influences of cofactors on syngas fermentation by C2~C6 acid/alcohol-producing Clostridium sp. JS66
- Genome analysis and influences of cofactors on syngas fermentation by C2~C6 acid/alcohol-producing Clostridium sp. JS66
- 이선미; 엄영순; 김경식; 최재표; 김경헌
- Issue Date
- 2018 한국생물공학회 추계학술발표대회 및 국제심포지엄
- Syngas and steel waste gas including carbon monoxide, carbon dioxide, and hydrogen are widely studied to use in biological production of acid and alcohol using acetogens. Among them, a newly isolated acetogen Clostridium sp. JS66 identified as a noble species with 33 % of DNA-DNA hybridization value with Clostridium carboxidivorans P7 is able to convert syngas into nC2~nC6 acid/alcohol metabolites through the Wood-Ljungdahl pathway and chain elongation pathways. Essential genes encoding formate dehydrogenase (Fdh) converting CO2 into formate, hydrogenase forming a tight complex with formate hydrogenase (Hyt), and aldehyde oxidoreductase (AOR) synthesizing aldehyde from acetate were identified through the genome analysis and were compared with the genes in Acetobacterium woodii and C. autoethanogenum. As those enzymes are known to need a specific metal ion such as tungsten (W), molybdenum (Mo) and selenium (Se) as a cofactor, the effects of Mo, W, and Se on syngas fermentation were investigated and elucidated with the genomic analysis. Further Omics study would provide more information on metabolic pathways and energetic conservation mechanism of Clostridium sp. JS66.
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