Biaxial Dielectrophoresis Force Spectroscopy: A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions

Title
Biaxial Dielectrophoresis Force Spectroscopy: A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions
Authors
이상엽남기환최정우박인수곽태준이규도손명구최승엽이세영장우진엄길호윤대성Rashid Bashir이상우
Keywords
dielectrophoresis force spectroscopy; intermolecular weak binding interactions; energy landscape; microfluidic device; pulling direction
Issue Date
2016-04
Publisher
ACS Nano
Citation
VOL 10, NO 4-4019
Abstract
The direct quantification of weak intermolecular binding interactions is very important for many applications in biology and medicine. Techniques that can be used to investigate such interactions under a controlled environment, while varying different parameters such as loading rate, pulling direction, rupture event measurements, and the use of different functionalized probes, are still lacking. Herein, we demonstrate a biaxial dielectrophoresis force spectroscopy (BDFS) method that can be used to investigate weak unbinding events in a high-throughput manner under controlled environments and by varying the pulling direction (i.e., transverse and/or vertical axes) as well as the loading rate. With the BDFS system, we can quantitatively analyze binding interactions related to hydrogen bonding or ionic attractions between functionalized microbeads and a surface within a microfluidic device. Our BDFS system allowed for the characterization of the number of bonds involved in an interaction, bond affinity, kinetic rates, and energy barrier heights and widths from different regimes of the energy landscape.
URI
http://pubs.kist.re.kr/handle/201004/64865
ISSN
1936-0851
Appears in Collections:
KIST Publication > Article
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