Protein stability changes of the novel p.Arg180Cys mutant A glycosyltransferase resulted in a weak A phenotype
- Protein stability changes of the novel p.Arg180Cys mutant A glycosyltransferase resulted in a weak A phenotype
- 이강봉; H.-S. Lee; K.-M. Choi; E. J. Won; M.-T. Thi Phan; S. Y. Lee; D.-J. Shin; S. Chun; G. Park; S.-K. Kim; H.-J. Lee; D. Cho
- blood groups; Genetics; genotyping; immunogenetics; RBC antigens and antibodies
- Issue Date
- Vox sanguinis
- VOL 111, NO 4-444
- A novel A subgroup allele (c.538C>T p.Arg180Cys) showing weak A phenotype was found in a 30-year-old Korean woman with ABO discrepancy. Using 3D structural analysis, protein stability prediction and flow cytometric analysis of ABO antigen expression on HeLa cells transfected with plasmids containing the p.Arg180Cys mutant, we found that the Arg180 residue in the loop region of the A glycosyltransferases (GTA) structure plays significant role in stabilizing its closed conformation, which is required for substrate binding and catalysis study.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.