Protein stability changes of the novel p.Arg180Cys mutant A glycosyltransferase resulted in a weak A phenotype

Title
Protein stability changes of the novel p.Arg180Cys mutant A glycosyltransferase resulted in a weak A phenotype
Authors
이강봉H.-S. LeeK.-M. ChoiE. J. WonM.-T. Thi PhanS. Y. LeeD.-J. ShinS. ChunG. ParkS.-K. KimH.-J. LeeD. Cho
Keywords
blood groups; Genetics; genotyping; immunogenetics; RBC antigens and antibodies
Issue Date
2016-11
Publisher
Vox sanguinis
Citation
VOL 111, NO 4-444
Abstract
A novel A subgroup allele (c.538C>T p.Arg180Cys) showing weak A phenotype was found in a 30-year-old Korean woman with ABO discrepancy. Using 3D structural analysis, protein stability prediction and flow cytometric analysis of ABO antigen expression on HeLa cells transfected with plasmids containing the p.Arg180Cys mutant, we found that the Arg180 residue in the loop region of the A glycosyltransferases (GTA) structure plays significant role in stabilizing its closed conformation, which is required for substrate binding and catalysis study.
URI
http://pubs.kist.re.kr/handle/201004/64893
ISSN
0042-9007
Appears in Collections:
KIST Publication > Article
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