In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes

Title
In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes
Authors
권익찬김광명류주희나진희이장욱박수빈조미희박성걸장현기강선웅김종호김병수박재형
Keywords
Vascular endothelial growth factor receptor; Matrix metalloproteinase; Molecular imaging; Angiogenesis; Fluorescence imaging
Issue Date
2016-07
Publisher
Amino acids
Citation
VOL 48, NO 7-1654
Abstract
Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-targeted in vivo imaging approach will be useful for understanding the detailed mechanism of angiogenesis and for evaluating therapeutic angiogenesis.
URI
http://pubs.kist.re.kr/handle/201004/64950
ISSN
0939-4451
Appears in Collections:
KIST Publication > Article
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