In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes
- In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes
- 권익찬; 김광명; 류주희; 나진희; 이장욱; 박수빈; 조미희; 박성걸; 장현기; 강선웅; 김종호; 김병수; 박재형
- Vascular endothelial growth factor
receptor; Matrix metalloproteinase; Molecular imaging; Angiogenesis; Fluorescence imaging
- Issue Date
- Amino acids
- VOL 48, NO 7-1654
- Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-targeted in vivo imaging approach will be useful for understanding the detailed mechanism of angiogenesis and for evaluating therapeutic angiogenesis.
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