Reducible Polyethylenimine Nanoparticles for Efficient siRNA Delivery in Corneal Neovascularization Therapy

Title
Reducible Polyethylenimine Nanoparticles for Efficient siRNA Delivery in Corneal Neovascularization Therapy
Authors
권익찬김광명김선화Hyounkoo HanSohee SonSejin SonNamho KimJi Young YheeJae Hyeop LeeJun-Sub ChoiChoun-Ki JooHohyeon LeeDuhwan LWon Jong KimHyuncheol Kim
Keywords
corneal neovascularization; nanoparticles; ocular delivery; reducible polyethylenimine; siRNA
Issue Date
2016-11
Publisher
Macromolecular bioscience
Citation
VOL 16-1597
Abstract
The aim of this study is to establish the safe and effective ocular delivery system of therapeutic small interfering RNA (siRNA) in corneal neovascularization therapy. The major hurdle present in siRNA-based corneal neovascularization (CNV) therapy is severe cytotoxicity caused by repetitive drug treatment. A reducible branched polyethylenimine (rBPEI)-based nanoparticle (NP) system is utilized as a new siRNA carrier as a hope for CNV therapy. The thiolated BPEI is readily self-crosslinked in mild conditions to make high molecular weight rBPEI thus allowing the creation of stable siRNA/rBPEI nanoparticles (siRNA-rBPEI-NPs). In the therapeutic region, the rBPEI polymeric matrix is effectively degraded into nontoxic LMW BPEI inside the reductive cytosol causing the rapid release of the encapsulated siRNA into the cytosol to carry out its function. The fluorescent-labeled siRNA-rBPEI-NPs can release siRNA into the entire corneal region after subconjuctival injection into the eye of Sprague Dawley rats thus confirming the proof of concept of this system.
URI
http://pubs.kist.re.kr/handle/201004/65156
ISSN
1616-5187
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KIST Publication > Article
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