Resonance Raman Probes for Organelle-Specific Labeling in Live Cells

Title
Resonance Raman Probes for Organelle-Specific Labeling in Live Cells
Authors
김세훈허정윤Andrey N. KuzminArtem PlissChang-Keun LimAlexander RzhevskiiBobo GuKen-Tye YongShangchun WenParas N. Prasad
Issue Date
2016-06
Publisher
Scientific Reports
Citation
VOL 6
Abstract
Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging.
URI
http://pubs.kist.re.kr/handle/201004/65164
ISSN
2045-2322
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE