Analysis of glycerol with isolation of endogenous interferences using "dilute and shoot”strategy and high-resolution mass spectrometry in human urine for antidoping testing

Title
Analysis of glycerol with isolation of endogenous interferences using "dilute and shoot”strategy and high-resolution mass spectrometry in human urine for antidoping testing
Authors
권오승이강미김호준이재익손정현박주형김기훈민호필성창민김용석
Keywords
Mass spectrometry; Doping control; Dilute and shoot; Orbitrap; Glycerol analysis
Issue Date
2016-12
Publisher
Mass Spectrometry Letters
Citation
VOL 7, NO 4-115
Abstract
Glycerol was identified and isolated from endogenous interferences during analysis of human urine using high-resolution mass spectrometry (HRMS) for doping control. Urinary sample preparation was simple; the samples were diluted with an organic solvent and then analyzed using a liquid chromatography-mass spectrometry (“dilute and shoot” method). Although the interfering ion peaks were observed at the similar retention time of glycerol, the inference could be identified by isolation with HRMS and further investigation. Thus, creatinine was identified as the endogenous interference for glycerol analysis and it also caused ion suppression resulting in the decrease of glycerol signal. This study reports the first identification and efficient isolation of endogenous interferences in human urine for “dilute and shoot” method. The information about ion suppression could be novel to prevent overestimation or a false result for antidoping analysis.
URI
http://pubs.kist.re.kr/handle/201004/65363
ISSN
2093-8950
Appears in Collections:
KIST Publication > Article
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