Evaluation of the Chemical Reporter Analog PNP-6AzGlcNAc as an O-GlcNAcase Substrate

Title
Evaluation of the Chemical Reporter Analog PNP-6AzGlcNAc as an O-GlcNAcase Substrate
Authors
남길수김은주John A HanoverMichelle R. Bond
Keywords
Metabolic chemical reporter; O-GlcNAcase; O-GlcNAc; PNP-6AzGlcNAc
Issue Date
2017-02
Publisher
Bulletin of the Korean Chemical Society
Citation
VOL 38, NO 2-270
Abstract
6-Azido-6-deoxy-N-acetylglucosamine (6AzGlcNAc) was recently introduced as a new selective metabolic chemical reporter (MCR) for labeling O-GlcNAc-modified proteins in cells. To investigate whether O-6AzGlcNAc is readily cleaved by O-GlcNAcase (OGA), the enzyme responsible for removing the OGlcNAc modification, we synthesized PNP-6AzGlcNAc. This analog mimics O-GlcNAc and can be used in vitro to define the kinetic parameters for OGA thereby defining whether O-6AzGlcNAc can be employed as an appropriate tool to monitor O-GlcNAc dynamics in cells. Both PNP-6AzGlcNAc and PNP-GlcNAc were efficiently hydrolyzed by OGA with similar kinetics suggesting that an azidomodification at the GlcNAc C6 position does not significantly interfere with the β-hexosaminidase activity of OGA.
URI
http://pubs.kist.re.kr/handle/201004/65434
ISSN
0253-2964
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KIST Publication > Article
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