Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles

Title
Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles
Authors
김병찬홍성길나현빈이진우윤종규정승욱이장원이병수김한솔Erik Hsiao김성환김병기박현규장호남현택환Jonathan S. DordickJay W. Grate김중배
Keywords
Enzymes; Enzyme catalysis; Single enzyme caged nanoparticles; Enzyme stabilization; Enzyme immobilization
Issue Date
2017-08
Publisher
Chemical engineering journal
Citation
VOL 322-515
Abstract
For the encapsulation of biomolecules in inorganic materials, we have developed a unique enzyme-silicate conjugate material that consists of a self-assembled molecularly thin silicate layer on the surface of each individual enzyme molecule. The enzyme-silicate conjugate materials, called single enzyme caged nanoparticles (SECNs), were synthesized via the silica polymerization on the surface of enzyme molecule after solubilizing each enzyme molecule in hexane by using a tiny amount of surfactant, called “ion-pairing”. SECNs possess near native enzyme activity in aqueous media with minimal substrate diffusional limitations, and are highly stable under the protection of silicate network cage. Due to their nearly molecular size, SECNs can also be adsorbed into mesoporous silica materials to yield robust and easily-recyclable enzymatic systems that can be used in a number of potential biocatalytic applications such as diagnostics, biosensors, biotransformations, biofuel production, bioremediation and CO2 capture.
URI
http://pubs.kist.re.kr/handle/201004/65642
ISSN
1385-8947
Appears in Collections:
KIST Publication > Article
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