Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles
- Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles
- 김병찬; 홍성길; 나현빈; 이진우; 윤종규; 정승욱; 이장원; 이병수; 김한솔; Erik Hsiao; 김성환; 김병기; 박현규; 장호남; 현택환; Jonathan S. Dordick; Jay W. Grate; 김중배
- Enzymes; Enzyme catalysis; Single enzyme caged nanoparticles; Enzyme stabilization; Enzyme immobilization
- Issue Date
- Chemical engineering journal
- VOL 322-515
- For the encapsulation of biomolecules in inorganic materials, we have developed a unique enzyme-silicate conjugate material that consists of a self-assembled molecularly thin silicate layer on the surface of each individual enzyme molecule. The enzyme-silicate conjugate materials, called single enzyme caged nanoparticles (SECNs), were synthesized via the silica polymerization on the surface of enzyme molecule after solubilizing each enzyme molecule in hexane by using a tiny amount of surfactant, called “ion-pairing”. SECNs possess near native enzyme activity in aqueous media with minimal substrate diffusional limitations, and are highly stable under the protection of silicate network cage. Due to their nearly molecular size, SECNs can also be adsorbed into mesoporous silica materials to yield robust and easily-recyclable enzymatic systems that can be used in a number of potential biocatalytic applications such as diagnostics, biosensors, biotransformations, biofuel production, bioremediation and CO2 capture.
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