A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip

Title
A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip
Authors
강민정박종민김조일노주윤김미라변재철
Keywords
Carbapenem; Antibiotic-susceptibility test; Laser desorption/ionization mass spectrometry; MALDI-TOF MS; Parylene-matrix chip
Issue Date
2017-09
Publisher
Enzyme and microbial technology
Citation
VOL 104-68
Abstract
A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDIMS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot, and day-today) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.
URI
http://pubs.kist.re.kr/handle/201004/65878
ISSN
0141-0229
Appears in Collections:
KIST Publication > Article
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