Optogenetic Modulation of Urinary Bladder Contraction for Lower Urinary Tract Dysfunction
- Optogenetic Modulation of Urinary Bladder Contraction for Lower Urinary Tract Dysfunction
- 신현준; 홍진기; 박재홍; 장자윤; 이규성; 강동묵; 서준교; Jieun An
- optogenetics; Urinary bladder; lower urinary tract dysfuction
- Issue Date
- Scientific Reports
- VOL 7-40872-13
- As current clinical approaches for lower urinary tract (LUT) dysfunction such as pharmacological and electrical stimulation treatments lack target specificity, thus resulting in suboptimal outcomes with various side effects, a better treatment modality with spatial and temporal target-specificity is necessary. In this study, we delivered optogenetic membrane proteins, such as channelrhodopsin-2 (ChR2) and halorhodopsin (NpHR), to bladder smooth muscle cells (SMCs) of mice using either the Creloxp transgenic system or a viral transfection method. The results showed that depolarizing ChR2-SMCs with blue light induced bladder contraction, whereas hyperpolarizing NpHR-SMCs with yellow light suppressed PGE2-induced overactive contraction. We also confirmed that optogenetic contraction of bladder smooth muscles in this study is not neurogenic, but solely myogenic, and that optogenetic light stimulation can modulate the urination in vivo. This study thus demonstrated the utility of optogenetic modulation of smooth muscle as a means to actively control the urinary bladder contraction with spatial and temporal accuracy. These features would increase the efficacy of bladder control in LUT dysfunctions without the side effects of conventional clinical therapies.
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