Efficient Direct Lineage Reprogramming of Fibroblasts into Induced Cardiomyocytes Using Nanotopographical Cues

Title
Efficient Direct Lineage Reprogramming of Fibroblasts into Induced Cardiomyocytes Using Nanotopographical Cues
Authors
김홍남Junsang YooYujung ChangHongwon KimSoonbong BaekHwan ChoiGun-Jae JeongJaein ShinByung-Soo KimJongpil Kim
Keywords
Direct Lineage Reprogramming; Induced Cardiomyocytes; Nanotopographical Cues
Issue Date
2017-03
Publisher
Journal of biomedical nanotechnology
Citation
VOL 13, NO 3-279
Abstract
Induced cardiomyocytes (iCMs) generated via direct lineage reprogramming offer a novel therapeutic target for the study and treatment of cardiac diseases. However, the efficiency of iCM generation is significantly low for therapeutic applications. Here, we show an efficient direct conversion of somatic fibroblasts into iCMs using nanotopographic cues. Compared with flat substrates, the direct conversion of fibroblasts into iCMs on nanopatterned substrates resulted in a dramatic increase in the reprogramming efficiency and maturation of iCM phenotypes. Additionally, enhanced reprogramming by substrate nanotopography was due to changes in the activation of focal adhesion kinase and specific histone modifications. Taken together, these results suggest that nanotopographic cues can serve as an efficient stimulant for direct lineage reprogramming into iCMs.
URI
http://pubs.kist.re.kr/handle/201004/65980
ISSN
1550-7033
Appears in Collections:
KIST Publication > Article
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