Efficient Direct Lineage Reprogramming of Fibroblasts into Induced Cardiomyocytes Using Nanotopographical Cues
- Efficient Direct Lineage Reprogramming of Fibroblasts into Induced Cardiomyocytes Using Nanotopographical Cues
- 김홍남; Junsang Yoo; Yujung Chang; Hongwon Kim; Soonbong Baek; Hwan Choi; Gun-Jae Jeong; Jaein Shin; Byung-Soo Kim; Jongpil Kim
- Direct Lineage Reprogramming; Induced Cardiomyocytes; Nanotopographical Cues
- Issue Date
- Journal of biomedical nanotechnology
- VOL 13, NO 3-279
- Induced cardiomyocytes (iCMs) generated via direct lineage reprogramming offer a novel therapeutic target for the study and treatment of cardiac diseases. However, the efficiency of iCM generation is significantly low for therapeutic applications. Here, we show an efficient direct conversion of somatic fibroblasts into iCMs using nanotopographic cues. Compared with flat substrates, the direct conversion of fibroblasts into iCMs on nanopatterned substrates resulted in a dramatic increase in the reprogramming efficiency and maturation of iCM phenotypes. Additionally, enhanced reprogramming by substrate nanotopography was due to changes in the activation of focal adhesion kinase and specific histone modifications. Taken together, these results suggest that nanotopographic cues can serve as an efficient stimulant for direct lineage reprogramming into iCMs.
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