Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells
- Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells
- 김홍남; Jangho Kim; Ki-Taek Lim; Yeonju Kim; Hoon Seonwoo; Soo Hyun Park; Hye Jin Lim; Deok-Ho Kim; Kahp-Yang Suh; Pill-Hoon Choung; Yun-Hoon Choung; Jong Hoon Chung
- Issue Date
- Scientific Reports
- VOL 3-3552-11
- Inspired by ultrastructural analysis of ex vivo human tissues as well as the physiological importance of structural density, we fabricated nanogrooves with 151, 153, and 155 spacing ratio (width5spacing, width5 550 nm). In response to the nanotopographical density, the adhesion, migration, and differentiation of human mesenchymal stem cells (hMSCs) were sensitively controlled, but the proliferation showed no significant difference. In particular, the osteo- or neurogenesis of hMSCs were enhanced at the 153 spacing ratio rather than 151 or 155 spacing ratio, implying an existence of potentially optimized nanotopographical density for stem cell function. Furthermore, such cellular behaviors were positively correlated with several cell morphological indexes as well as the expression of integrin b1 or N-cadherin. Our findings propose that nanotopographical density may be a key parameter for the design and manipulation of functional scaffolds for stem cell-based tissue engineering and regenerative medicine.
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