Genetically Engineered Phage Induced Selective H9c3 Cardiomyocytes Patterning in PDMS Microgrooves

Title
Genetically Engineered Phage Induced Selective H9c3 Cardiomyocytes Patterning in PDMS Microgrooves
Authors
전호정권청아김용준
Keywords
PDMS; micropatterns; RGD-phage; cell-surface interaction; cardiomyocytes
Issue Date
2017-08
Publisher
Materials
Citation
VOL 10, NO 8-973-8
Abstract
A micro-patterned cell adhesive surface was prepared for future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro-patterns were prepared by a photolithography process. Afterwards, recombinant filamentous phages that displayed a short binding motif with a cell adhesive peptide (-RGD-) on p8 proteins were immobilized on PDMS microgrooves through simple contact printing to study the cellular response of rat H9c2 cardiomyocyte. While the cell density decreased on PDMS micro-patterns, we observed enhanced cell proliferation and cell to surface interaction on the RGD-phage coated PDMS microgrooves. The RGD-phage coating also supported a better alignment of cell spreading rather than isotropic cell growths as we observed on non-pattered PDMS surface.
URI
http://pubs.kist.re.kr/handle/201004/66095
ISSN
1996-1944
Appears in Collections:
KIST Publication > Article
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