Identifying methanogen community structures and their correlations with performance parameters in four full-scale anaerobic sludge digesters

Title
Identifying methanogen community structures and their correlations with performance parameters in four full-scale anaerobic sludge digesters
Authors
조경진황석환구태완신승구이준엽한규성김웅
Keywords
Anaerobic digestion; 454 pyrosequencing; Real-time quantitative PCR; Archaea-to-bacteria ratio; Methanoculleus; Methanoregula; Biomarker
Issue Date
2017-03
Publisher
Bioresource technology
Citation
VOL 228-373
Abstract
Four full-scale mesophilic anaerobic digesters treating waste sludge were monitored to characterize methanogen communities and their relationship with process parameters. The performance of the four digesters were dissimilar with the average chemical oxygen demand removal efficiencies between 24 and 45% and differing pH. Real-time quantitative PCR showed that archaeal 16S rRNA gene concentration ([ARC]) and, more pronouncedly, its ratio to bacterial counterpart ([ARC]/[BAC]) correlated positively with the performance parameters, including the lipid removal efficiency. Pyrosequencing identified 12 methanogen genera, of which Methanolinea, Methansaeta, and Methanospirillum collectively accounted for 79.2% of total archaeal reads. However, Methanoculleus, a numerically minor (1.9 ± 2.6%) taxa, was the most promising biomarker for positive performance, while Methanoregula was abundant in samples with poor performance. These results could be useful for the control and management of anaerobic sludge digestion.
URI
http://pubs.kist.re.kr/handle/201004/66117
ISSN
0960-8524
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE