Multiplex real-tiem PCR using encoded micro particles for microRNA profiling

Title
Multiplex real-tiem PCR using encoded micro particles for microRNA profiling
Authors
김상경Seungwon Jung
Keywords
Encoded particle; Hydrogel; MicroRNA; Multiplex; Real-time PCR
Issue Date
2017-08
Publisher
functional genomics, Methods molecular biology vol. 1654
Citation
VOL 1654-230
Abstract
Multiplex quantitative real-time PCR (qPCR), which measures multiple DNAs in a given sample, has drawn unprecedented attention as a means of verifying the rapidly increasing genetic targets in a single phenotype. We report the detailed procedure of a readily extensible qPCR for multiple microRNA (miRNA) expression analysis using microparticles of primer-immobilized networks as discrete reactors. Individual particles are identified by two-dimensional codes engraved into the particles. It allows high-fidelity signal analysis in the multiplex real-time PCR. During the course of PCR, the amplicons accumulate in the volume of the particles with amplification efficiency over 95%. Tens of miRNAs can be quantitatively profiled in a single PCR reaction of this method.
URI
http://pubs.kist.re.kr/handle/201004/66295
ISSN
9781-4939
Appears in Collections:
KIST Publication > ETC
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE