An improvement of miRNA extraction efficiency in human plasma

Title
An improvement of miRNA extraction efficiency in human plasma
Authors
유영숙송은주채동규Eunmi Ban
Keywords
miRNA; glycogen; yeast tRNA; extraction effieicney
Issue Date
2017-11
Publisher
Analytical and bioanalytical chemistry
Citation
VOL 409, NO 27-6404
Abstract
MicroRNAs (miRNAs) are short noncoding RNA molecules that control the expression of mRNAs associated with various biological processes. Therefore, deregulated miRNAs play important roles in the pathogenesis of diseases. Numerous studies are aimed at discovering biomarkers of diseases or determining miRNA functions by monitoring circulating miRNAs in various biological sources such as plasma and urine. However, the analysis of miRNA in such fluids presents problems related to accuracy and reproducibility because of their low levels in biological fluids. Therefore, better extraction kits and more sensitive detection systems have been developed for improved and reproducible analysis of circulating miRNAs. However, new extraction methods are also needed to improve the yield of miRNAs for their reliable analysis from biological fluids. The combination of yeast transfer RNA (tRNA) and glycogen as carrier molecules and incubation durations were optimized to maximize extraction efficiency. The extraction recovery using a combination of yeast tRNA and glycogen was approximately threefold more than that by using glycogen or yeast tRNA alone. In addition, reproducible and accurate analysis of miRNAs can be carried out after extraction using a combination of yeast tRNA and glycogen without an impact on plasma components.
URI
http://pubs.kist.re.kr/handle/201004/66311
ISSN
1618-2642
Appears in Collections:
KIST Publication > Article
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