Physiological Effects of Ac4ManNAz and Optimization of Metabolic Labeling for Cell Tracking

Title
Physiological Effects of Ac4ManNAz and Optimization of Metabolic Labeling for Cell Tracking
Authors
김광명한상수이동은심혜은이상민정택희오정화이향애문성환전종호윤석주강선웅
Keywords
Metabolic labeling; Azido-sugar; Modified glycosylation; Azido group; Physiology
Issue Date
2017-03
Publisher
Theranostics
Citation
VOL 7, NO 5-1176
Abstract
Metabolic labeling techniques are powerful tools for cell labeling, tracking and proteomic analysis.However, at present, the effects of the metabolic labeling agents on cell metabolism and physiology are not known. To address this question, in this study, we analyzed the effects of cells treated with Ac4ManNAz through microarray analysis and analyses of membrane channel activity, individual bio-physiological properties, and glycolytic flux. According to the results, treatment with 50 μM Ac4ManNAz led to the reduction of major cellular functions, including energy generation capacity, cellular infiltration ability and channel activity. Interestingly, 10 μM Ac4ManNAz showed the least effect on cellular systems and had a sufficient labeling efficiency for cell labeling, tracking and proteomic analysis. Based on our results, we suggest 10 μM as the optimum concentration of Ac4ManNAz for in vivo cell labeling and tracking. Additionally, we expect that our approach could be used for cell-based therapy for monitoring the efficacy of molecule delivery and the fate of recipient cells.
URI
http://pubs.kist.re.kr/handle/201004/66381
ISSN
1838-7640
Appears in Collections:
KIST Publication > Article
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