SDF-1 peptide tethered polyester facilitates tissue repair by endogenous cell mobilization and recruitment
- SDF-1 peptide tethered polyester facilitates tissue repair by endogenous cell mobilization and recruitment
- 김수현; Muhammad Shafiq; Deling Kong
- stem cell recruitment; in situ tissue regeneration; vascular graft; SDF1-a; polyester
- Issue Date
- Journal of biomedical materials research. Part A
- VOL 105, NO 10-2684
- The design of bioactive scaffolds that can invoke host's own regenerative capabilities and facilitate endogenous tissue repair hold great promise. This study aims to evaluate the potential of stromal cell-derived factor 1 alpha (SDF-1)-derived peptide and heparin tethered poly(L-lactide-co-epsilon-caprolactone) (PLCL) copolymers for blood vessel regeneration applications. Amino acid analysis and toluidine blue assays confirm successful conjugation of SDF-1 peptide and heparin with the PLCL copolymers. Assessment of biocompatibility after subcutaneous implantation in rats discloses higher cell infiltration in SDF-1 peptide (SDF-1 group) or SDF-1 peptide and heparin (SDF-1/heparin group) than the control group. SDF-1 and SDF-1/heparin grafts also show more numbers of laminin(+) blood vessels, CD90(+) stem cells, and alpha smooth muscle actin(+) cells than the control group. However, SDF-1 and SDF-1/heparin groups did not significantly differ in terms of blood vessel regeneration and stem cell recruitment. Evaluation of the inflammatory response reveal less numbers of CD68(+) macrophages in SDF-1 and SDF-1/heparin groups compared with the control group; whereas three groups show similar numbers of CD206(+) macrophages. These results indicate that completely synthetic, cell-free grafts can attract endogenous cells and enhance tissue repair. Bioactive polyesters can be fabricated into different shapes and structures for various tissue engineering applications.
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