Fabrication of micrometer-scale porous gelatin scaffolds for 3D cell culture

Title
Fabrication of micrometer-scale porous gelatin scaffolds for 3D cell culture
Authors
정영미최낙원김태희심규영김소현이동욱김범상성종환
Keywords
Gelatin-methacrylate (Gel-MA); Micrometer-scale porous hydrogel scaffold; Photocrosslinking; Porogen; 3D cell culture
Issue Date
2017-06
Publisher
Journal of industrial and engineering chemistry
Citation
VOL 50-189
Abstract
In hydrogel scaffolds for 3D cell culture, porous structure plays a central role in maintaining cell survival by delivery of biochemical factors through the scaffolds. Thus, adequate pore structures should be a significant design factor for preparing cell culture scaffolds. Here, we present a non-cytotoxic approach to control porosity of a gelatin scaffold based on porogen leaching. Specifically, we fabricated photocrosslinkable hydrogel (gelatin methacrylate) containing porogens in a microfluidic channel, followed by dissolving out the porogens. Our approach allowed generation of micrometer-scale pores, increasing cell viability. This study could serve as a guide for tuning porosity of cell-seeded 3D scaffolds.
URI
http://pubs.kist.re.kr/handle/201004/66623
ISSN
1226-086X
Appears in Collections:
KIST Publication > Article
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