The C-terminus of IGFBP-5 suppresses tumor growth by inhibiting angiogenesis

Title
The C-terminus of IGFBP-5 suppresses tumor growth by inhibiting angiogenesis
Authors
안형준황재령조영재이윤나박영미한희동이정원Je-Ho Lee
Keywords
IGFBP-5; tumor suppressor; ovarian cancer
Issue Date
2016-12
Publisher
Scientific Reports
Citation
VOL 6-39334-12
Abstract
Insulin-like growth factor-binding protein 5 (IGFBP-5) plays a role in cell growth, differentiation, and apoptosis. In this study, we found that IGFBP5 was markedly downregulated in ovarian cancer tissue. We investigated the functional significance of IGFBP-5 as a tumor suppressor. To determine functional regions of IGFBP-5, truncation mutants were prepared and were studied the effect on tumor growth. Expression of C-terminal region of IGFBP-5 significantly decreased tumor growth in an ovarian cancer xenograft. A peptide derived from the C-terminus of IGFBP-5 (BP5-C) was synthesized to evaluate the minimal amino acid motif that retained anti-tumorigenic activity and its effect on angiogenesis was studied. BP5-C peptide decreased the expression of VEGF-A and MMP-9, phosphorylation of Akt and ERK, and NF-kB activity, and inhibited angiogenesis in in vitro and ex vivo systems. Furthermore, BP5-C peptide significantly decreased tumor weight and angiogenesis in both ovarian cancer orthotopic xenograft and patient-derived xenograft mice. These results suggest that the C-terminus of IGFBP-5 exerts anti-cancer activity by inhibiting angiogenesis via regulation of the Akt/ERK and NF-kB-VEGF/MMP-9 signaling pathway, and might be considered as a novel angiogenesis inhibitor for the treatment of ovarian cancer.
URI
http://pubs.kist.re.kr/handle/201004/66938
ISSN
2045-2322
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KIST Publication > Article
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