Mapping cell behavior across a wide range of vertical silicon nanocolumn densities

Title
Mapping cell behavior across a wide range of vertical silicon nanocolumn densities
Authors
이경은Nina Buch-MansonDong-Hee KangDongyoon KimMyung-Han YoonKaren L. Martinez
Issue Date
2017-05
Publisher
Nanoscale
Citation
VOL 9, NO 17-5527
Abstract
Over the past decade, vertical nanostructures have provided novel approaches for biomedical applications such as intracellular delivery/detection, specific cell capture, membrane potential measurement, and cellular activity regulation. Although the feasibility of the vertical nanostructures as a new biological tool has been thoroughly demonstrated, a better understanding of cell behavior on vertical nanostructures, in particular the effects of geometry, is essential for advanced applications. To investigate the cell behavior according to the variation of the spacing between vertical nanostructures, we have interfaced fibroblasts (NIH3T3) with density-controlled vertical silicon nanocolumn arrays (vSNAs). Over a wide range of vSNA densities, we observe three distinct cell settling regimes and investigate both overall cell behavior (adhesions, morphology, and mobility) and detailed biomacromolecule variance (F-actin and focal adhesion) across these regimes. We expect that these systematic observations could serve as a guide for improved nanostructure array design for the desired cell manipulation.
URI
http://pubs.kist.re.kr/handle/201004/66961
ISSN
2040-3364
Appears in Collections:
KIST Publication > Article
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