Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement

Title
Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement
Authors
엄영순윤성규주영철강대희신상규현정은우한민박철환한성옥
Keywords
fatty acids production; Saccharomyces cerevisiae; nitrogen optimization; cofactor balancing; animal feed supplement
Issue Date
2017-12
Publisher
Journal of agricultural and food chemistry
Citation
VOL 65, NO 50-11035
Abstract
Saccharomyces cerevisiae is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but S. cerevisiae produces small amounts of fatty acids. In this study, we enhanced fatty acid production of S. cerevisiae by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wildtype strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of S. cerevisiae.
URI
http://pubs.kist.re.kr/handle/201004/67160
ISSN
0021-8561
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE