Identification of plasma membrane glycoproteins specific to human glioblastoma multiforme cells using lectin arrays and LC-MS/MS

Title
Identification of plasma membrane glycoproteins specific to human glioblastoma multiforme cells using lectin arrays and LC-MS/MS
Authors
이철주한기철이지은박예은염정훈김영수이혜진이승택
Keywords
plasma membrane glycoprotein; lectin array; mass spectrometry; biomarker
Issue Date
2018-01
Publisher
Proteomics
Citation
VOL 18, NO 1-1700302-14
Abstract
Glioblastoma, also known as glioblastoma multiforme (GBM), is the most malignant type of brain cancer and has poor prognosis with a median survival of less than one year. While the structural changes of tumor cell surface carbohydrates are known to be associated with invasive behavior of tumor cells, the cell surface glycoproteins to differentiate the low- and high-grade glioma cells can be potential diagnostic markers and therapeutic targets for GBMs. In the present study, lectin arrays consisting of eight lectins were employed to explore cell surface carbohydrate expression patterns on low-grade oligodendroglioma cells (Hs683) and GBM cells (T98G). Griffonia simplicifolia I (GS I) was found to selectively bind to T98G cells and not to Hs683 cells. For identification of the glioblastoma-specific cell surface markers, the glycoproteins from each cell type were captured by a GS I lectin column and analyzed by LC-MS/MS. The identified proteins from the two cell types were quantified using label-free quantitative analysis based on spectral counting. Of cell surface glycoproteins showing significant increases in T98G cells, five proteins were selected for verification of both protein and glycosylation level changes using Western blot and GS I lectin-based immunosorbent assay.
URI
http://pubs.kist.re.kr/handle/201004/67222
ISSN
1615-9853
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE