High-throughput and direct measurement of androgen levels using turbulent flow chromatography liquid chromatography-triple quarupole mass spectrometry (TFC-LC-TQMS) to discover chemicals that modulate dihydrotestosterone production in human prostate cancer celss

Title
High-throughput and direct measurement of androgen levels using turbulent flow chromatography liquid chromatography-triple quarupole mass spectrometry (TFC-LC-TQMS) to discover chemicals that modulate dihydrotestosterone production in human prostate cancer celss
Authors
이희주이은하강경수판철호최용수김상민정유진김주연펭 레이Sang Hyun Sung
Keywords
5a-reductase inhibitor; Dihydrotestosterone; Fucoxanthin; Naringenin; Testosterone
Issue Date
2018-02
Publisher
Biotechnology letters
Citation
VOL 40, NO 2-270
Abstract
Objectives To develop a high-throughput screening system to measure the conversion of testosterone to dihydrotestosterone (DHT) in cultured human prostate cancer cells using turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS). Results After optimizing the cell reaction system, this method demonstrated a screening capability of 103 samples, including 78 single compounds and 25 extracts, in less than 12 h without manual sample preparation. Consequently, fucoxanthin, phenethyl caffeate, and Curcuma longa L. extract were validated as bioactive chemicals that inhibited DHT production in cultured DU145 cells. In addition, naringenin boosted DHT production in DU145 cells. Conclusion The method can facilitate the discovery of bioactive chemicals that modulate the DHT production, and four phytochemicals are potential candidates of nutraceuticals to adjust DHT levels in male hormonal dysfunction.
URI
http://pubs.kist.re.kr/handle/201004/67330
ISSN
0141-5492
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KIST Publication > Article
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